Advancements in Synthetic Biology for The Development of Innovative Laboratory Diagnostic Tools: A Comprehensive Review

Abstract

Background: Synthetic biology has emerged as a transformative field with the potential to address global challenges in health, sustainability, and resource accessibility. This review explores the advancements in synthetic biology aimed at developing new laboratory diagnostic tools. We analyze the methodologies employed in both cell-based and cell-free systems, emphasizing their applicability in diverse environments, including resource-limited and off-the-grid settings.

Methods: The review synthesizes findings from recent studies demonstrating the successful implementation of synthetic biology techniques in real-world scenarios, such as bioproduction, biosensing, and therapeutic delivery.

Results: Results indicate that while whole-cell platforms offer advantages in mass production and complexity, cell-free systems provide enhanced stability and flexibility for rapid diagnostics. However, both approaches face significant challenges, including genetic stability, resource requirements, and operational complexity. The review highlights the necessity of interdisciplinary approaches to overcome these obstacles and improve the robustness and usability of synthetic biology applications.

Conclusion: Ultimately, we conclude that continued innovation in synthetic biology can lead to the creation of effective diagnostic tools that are not only responsive to immediate medical needs but also sustainable for future applications in remote and resource-limited contexts.